HCT-116
HCT-116
规格:
货期:
编号:TS259901
品牌:Testobio
| 产品名称： | HCT-116 |
|---|---|
| 商品货号： | TS259901 |
| <strong>Cell line </strong>： | HCT-116 |
| DSMZ no. ： | TS259901 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary colon carcinoma of an adult man; cells were described in the literature to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice |
| Reference(s) ： | 14406, 14405 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like small cells growing as monolayer; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS + 2 mM L-glutamine (alternatively Dulbeccos MEM with high glucose) |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:3 to 1:6 about 2-3 x per week using trypsin/EDTA; in confluent cultures with high densities, cells may detach spontaneously |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-40 hours |
| Harvest ： | cell harvest of ca. 10-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | HCT-116 |
|---|---|
| 商品货号： | TS259901 |
| <strong>Cell line </strong>： | HCT-116 |
| DSMZ no. ： | TS259901 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary colon carcinoma of an adult man; cells were described in the literature to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice |
| Reference(s) ： | 14406, 14405 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like small cells growing as monolayer; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS + 2 mM L-glutamine (alternatively Dulbeccos MEM with high glucose) |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:3 to 1:6 about 2-3 x per week using trypsin/EDTA; in confluent cultures with high densities, cells may detach spontaneously |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-40 hours |
| Harvest ： | cell harvest of ca. 10-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |
