OCI-AML3
OCI-AML3
规格:
货期:
编号:TS259902
品牌:Testobio
| 产品名称： | OCI-AML3 |
|---|---|
| 商品货号： | TS259902 |
| <strong>Cell line </strong>： | OCI-AML3 |
| DSMZ no. ： | TS259902 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4) at diagnosis in 1987; cells carry an NPM1 gene mutation (type A) and the DNMT3A R882C mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14870, 15050, 15051, 16666, 16667, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, mostly round cells growing in suspension; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 48(45-50)<2n>X/XY, +1, +5, +8, der(1)t(1;18)(p11;q11), i(5p), del(13)(q13q21), dup(17)(q21q25) - sideline with r(Y)x1-2 - hemizygous for RB1 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | OCI-AML3 |
|---|---|
| 商品货号： | TS259902 |
| <strong>Cell line </strong>： | OCI-AML3 |
| DSMZ no. ： | TS259902 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4) at diagnosis in 1987; cells carry an NPM1 gene mutation (type A) and the DNMT3A R882C mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14870, 15050, 15051, 16666, 16667, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, mostly round cells growing in suspension; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 48(45-50)<2n>X/XY, +1, +5, +8, der(1)t(1;18)(p11;q11), i(5p), del(13)(q13q21), dup(17)(q21q25) - sideline with r(Y)x1-2 - hemizygous for RB1 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
