NU-DUL-1
NU-DUL-1
规格:
货期:
编号:TS259906
品牌:Testobio
| 产品名称： | NU-DUL-1 |
|---|---|
| 商品货号： | TS259906 |
| <strong>Cell line </strong>： | NU-DUL-1 |
| DSMZ no. ： | TS259906 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1982 from the cerebrospinal fluid of a 43-year-old Caucasian man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as undifferentiated lymphoma; assigned to ABC-like lymphoma subtype (activated B-cell) |
| Reference(s) ： | 14517, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing singly in suspension |
| Medium ： | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3 days; maintain at ca. 0.5-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-36 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 45<2n>X, -Y, der(X)t(X;13)(p11.2;q13), del(3)(q26-28), der(5)t(X;5)(q26;q21), del(6)(p21p22), del(6)(q22), der(8)t(8;14)(q24;q32), add(11)(q23-24), der(14)t(8;14)(q24;q32)t(14;13)(q32;?q14)t(13;X)(?q13;p11.2), der(17)t(17;18)(q22;q21.1), der(18)t(5;18)(q21;q21.1)inv(5)(q21;q35), der(19)t(?17;19)(q22;q13.2) - carries semi-cryptic t(8;14) effecting IGH-MYC rearrangement - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | NU-DUL-1 |
|---|---|
| 商品货号： | TS259906 |
| <strong>Cell line </strong>： | NU-DUL-1 |
| DSMZ no. ： | TS259906 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1982 from the cerebrospinal fluid of a 43-year-old Caucasian man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as undifferentiated lymphoma; assigned to ABC-like lymphoma subtype (activated B-cell) |
| Reference(s) ： | 14517, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing singly in suspension |
| Medium ： | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3 days; maintain at ca. 0.5-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-36 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 45<2n>X, -Y, der(X)t(X;13)(p11.2;q13), del(3)(q26-28), der(5)t(X;5)(q26;q21), del(6)(p21p22), del(6)(q22), der(8)t(8;14)(q24;q32), add(11)(q23-24), der(14)t(8;14)(q24;q32)t(14;13)(q32;?q14)t(13;X)(?q13;p11.2), der(17)t(17;18)(q22;q21.1), der(18)t(5;18)(q21;q21.1)inv(5)(q21;q35), der(19)t(?17;19)(q22;q13.2) - carries semi-cryptic t(8;14) effecting IGH-MYC rearrangement - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |
