VAL
VAL
规格:
货期:
编号:TS259910
品牌:Testobio
| 产品名称： | VAL |
|---|---|
| 商品货号： | TS259910 |
| <strong>Cell line </strong>： | VAL |
| DSMZ no. ： | TS259910 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | derived from the bone marrow of a 50-year-old woman with B-acute lymphoblastic leukemia (B-ALL) in 1985; cells were described in the literature to carry the three-way-translocation t(8;14;18) and to overexpress the BCL2 and MYC genes. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14397, 14442, 14498, 14657, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph small cells, growing singly or in clumps in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 48(44-50)<2n>XX, +8, +12, t(3;4)(q27;p11), der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), ider(8)(q10)t(8;14)(q24;q32)t(14;18)(q32;q21), der(12)t(?1;12)(q25;q24), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - sideline with dup(2)(p1?p2?) - carries rearrangements of BCL2, BCL6, IGH and MYC - resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
VAL
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | VAL |
|---|---|
| 商品货号： | TS259910 |
| <strong>Cell line </strong>： | VAL |
| DSMZ no. ： | TS259910 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | derived from the bone marrow of a 50-year-old woman with B-acute lymphoblastic leukemia (B-ALL) in 1985; cells were described in the literature to carry the three-way-translocation t(8;14;18) and to overexpress the BCL2 and MYC genes. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14397, 14442, 14498, 14657, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph small cells, growing singly or in clumps in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 48(44-50)<2n>XX, +8, +12, t(3;4)(q27;p11), der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), ider(8)(q10)t(8;14)(q24;q32)t(14;18)(q32;q21), der(12)t(?1;12)(q25;q24), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - sideline with dup(2)(p1?p2?) - carries rearrangements of BCL2, BCL6, IGH and MYC - resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
