RH-41
RH-41
规格:
货期:
编号:TS259922
品牌:Testobio
| 产品名称： | RH-41 |
|---|---|
| 商品货号： | TS259922 |
| <strong>Cell line </strong>： | RH-41 |
| DSMZ no. ： | TS259922 |
| Species ： | confirmed originally as human by species PCR and later by STR fingerprinting |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from the alveololar rhabdomyosarcoma of the liver of a girl after chemotherapy; cells were described in the literature to contain a deletion mutation of p53 and to be resistant to FAS- and TRAIL-induced apoptosis |
| Reference(s) ： | 14848, 15010 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:10 to 1:20 once a week using trypsin/EDTA; optimal density at about 20 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | cell harvest of ca. 40 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | RH-41 |
|---|---|
| 商品货号： | TS259922 |
| <strong>Cell line </strong>： | RH-41 |
| DSMZ no. ： | TS259922 |
| Species ： | confirmed originally as human by species PCR and later by STR fingerprinting |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from the alveololar rhabdomyosarcoma of the liver of a girl after chemotherapy; cells were described in the literature to contain a deletion mutation of p53 and to be resistant to FAS- and TRAIL-induced apoptosis |
| Reference(s) ： | 14848, 15010 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:10 to 1:20 once a week using trypsin/EDTA; optimal density at about 20 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | cell harvest of ca. 40 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
