JIYOYE
JIYOYE
规格:
货期:
编号:TS259926
品牌:Testobio
| 产品名称： | JIYOYE |
|---|---|
| 商品货号： | TS259926 |
| <strong>Cell line </strong>： | JIYOYE |
| DSMZ no. ： | TS259926 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | cell line JIYOYE (also known as JIJOYE, P-2003 or P-3) was established from a 7-year-old black African male with Burkitt lymphoma in 1965 |
| Reference(s) ： | 14683, 14863 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoid cells growing in suspension, singly or in clumps |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5-0.8 x 106 cells/ml in 24-well-plates in 20% FBS; after recovery, viability may be low! maintain at ca. 0.3-1.0 x 106 cells/ml; maximum density at ca. 2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-60 hours |
| Harvest ： | maximum density at about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda - |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 13% polyploidy - 42-48<2n>XY, der(7)t(1;7)(q32;q32), t(8;14)(q24;q32), i(9p), der(14)t(9;14)(q11;q32), +0-2mar; carries t(8;14) rearranging IGH with MYC (far upstream breakpoint) |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
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周雪
13372109301
| 产品名称： | JIYOYE |
|---|---|
| 商品货号： | TS259926 |
| <strong>Cell line </strong>： | JIYOYE |
| DSMZ no. ： | TS259926 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | cell line JIYOYE (also known as JIJOYE, P-2003 or P-3) was established from a 7-year-old black African male with Burkitt lymphoma in 1965 |
| Reference(s) ： | 14683, 14863 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoid cells growing in suspension, singly or in clumps |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5-0.8 x 106 cells/ml in 24-well-plates in 20% FBS; after recovery, viability may be low! maintain at ca. 0.3-1.0 x 106 cells/ml; maximum density at ca. 2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-60 hours |
| Harvest ： | maximum density at about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda - |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 13% polyploidy - 42-48<2n>XY, der(7)t(1;7)(q32;q32), t(8;14)(q24;q32), i(9p), der(14)t(9;14)(q11;q32), +0-2mar; carries t(8;14) rearranging IGH with MYC (far upstream breakpoint) |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
