L-82
L-82
规格:
货期:
编号:TS259933
品牌:Testobio
| 产品名称： | L-82 |
|---|---|
| 商品货号： | TS259933 |
| <strong>Cell line </strong>： | L-82 |
| DSMZ no. ： | TS259933 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | anaplastic large cell lymphoma |
| Origin ： | established from the pleural effusion of a 24-year-old woman with recurrent anaplastic large cell lymphoma (ALCL); cells were described in the literature to clonally derive from T-lineage lymphoid cells and to be tumorigenic in SCID mice |
| Reference(s) ： | 14761 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing singly in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; maintain at ca. 0.3-1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximum density at about 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | cy/smCD3 -, CD4 +, CD5 +, CD6 -, CD7 -, CD8 -, CD11b -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD71 +, TRCalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded karyotype with 4% polyploidy 72-77<3n>XX/XXX,+1,+2,+5,+7,+9,+10,-14,-15,+4-6mar; add(X)(q12), add(X)(q27), add(1)(p11), t(2;5)(p23;q35), der(2)t(2;5)(p23;q35), der(4)add(4)(p14q35), der(6)i(6)(q10)t(1;6)(q41;p24), add(7)(q22), add(8)(q24), add(9)(p2?2)x2, add(9)(q11), add(10)(p1?), add(13)(q33), der(13)t(13;15)(p11;q14), del(16)(q23), add(17)(p1?); carries t(2;5) effecting NPM1-ALK rearrangement, and 7q22 amplification targeting CDK6 upregulation; matches published karyotype |
| Molec. Genetics ： | expression of fusion gene NPM1-ALK confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | L-82 |
|---|---|
| 商品货号： | TS259933 |
| <strong>Cell line </strong>： | L-82 |
| DSMZ no. ： | TS259933 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | anaplastic large cell lymphoma |
| Origin ： | established from the pleural effusion of a 24-year-old woman with recurrent anaplastic large cell lymphoma (ALCL); cells were described in the literature to clonally derive from T-lineage lymphoid cells and to be tumorigenic in SCID mice |
| Reference(s) ： | 14761 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing singly in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; maintain at ca. 0.3-1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximum density at about 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | cy/smCD3 -, CD4 +, CD5 +, CD6 -, CD7 -, CD8 -, CD11b -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD71 +, TRCalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded karyotype with 4% polyploidy 72-77<3n>XX/XXX,+1,+2,+5,+7,+9,+10,-14,-15,+4-6mar; add(X)(q12), add(X)(q27), add(1)(p11), t(2;5)(p23;q35), der(2)t(2;5)(p23;q35), der(4)add(4)(p14q35), der(6)i(6)(q10)t(1;6)(q41;p24), add(7)(q22), add(8)(q24), add(9)(p2?2)x2, add(9)(q11), add(10)(p1?), add(13)(q33), der(13)t(13;15)(p11;q14), del(16)(q23), add(17)(p1?); carries t(2;5) effecting NPM1-ALK rearrangement, and 7q22 amplification targeting CDK6 upregulation; matches published karyotype |
| Molec. Genetics ： | expression of fusion gene NPM1-ALK confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
