DM-3
DM-3
规格:
货期:
编号:TS259934
品牌:Testobio
| 产品名称： | DM-3 |
|---|---|
| 商品货号： | TS259934 |
| <strong>Cell line </strong>： | DM-3 |
| DSMZ no. ： | TS259934 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | sarcomatoid mesothelioma |
| Origin ： | established from a pleural biopsy of a 62-year-old man with malignant mesothelioma |
| Reference(s) ： | 14850 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, sarcomatoid cells growing in monolayers; image; image ; image |
| Medium ： | 80% Dulbeccos MEM or NCTC-109 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:4 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109) |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype. |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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DM-3
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周雪
13372109301
| 产品名称： | DM-3 |
|---|---|
| 商品货号： | TS259934 |
| <strong>Cell line </strong>： | DM-3 |
| DSMZ no. ： | TS259934 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | sarcomatoid mesothelioma |
| Origin ： | established from a pleural biopsy of a 62-year-old man with malignant mesothelioma |
| Reference(s) ： | 14850 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, sarcomatoid cells growing in monolayers; image; image ; image |
| Medium ： | 80% Dulbeccos MEM or NCTC-109 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:4 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109) |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype. |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
