KMS-12-PE
KMS-12-PE
规格:
货期:
编号:TS259955
品牌:Testobio
| 产品名称： | KMS-12-PE |
|---|---|
| 商品货号： | TS259955 |
| <strong>Cell line </strong>： | KMS-12-PE |
| DSMZ no. ： | TS259955 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | multiple myeloma |
| Origin ： | established in 1987 from the pleural effusion of a 64-year-old woman with refractory, terminal multiple myeloma (Ig-non-producing) after combination chemotherapy; described in the literature to carry t(11:14)(q13:q32) rearrangement; sister cell line of KMS-12-BM (DSM ACC 551) |
| Reference(s) ： | 14799, 14817, 14818 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or occasionally in small clumps in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | optimal split ratio of 1:2 to 1:4 every 2-5 days; maintain at 0.5-1.0 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml; cells should be cultured in 24-well plates to maximum density at ca. 2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | maximum density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD34 -, CD38 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile (identical to that of sister cell line KMS-12-BM; ACC 551) |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy; 42(40-47)<2n>X,-X, +1, +7, -14, +16, +18, -22, der(1;16)(q10;p10), der(1)(1pter->q12::18q1?-q2?1::14q21.2->q32.32::11q.13->qter)qdp(1q12.1->q12::18q1?->q21), der(3)dup(3)(q26q29)t(3;21)(q29;q11.2), del(4)(q26), der(5)t(5;17)(p10;q10), der(6)t(1;6)(q32;q22), der(7)t(7;10)(p21;p13), der(8)ins(8;14)(q24;q32.3q32.3)x2, ider(9)(q10)t(9;11)(q24;q11), der(10;16)(q10;q10), der(11)t(11;14)(q13;q32), der(11)t(9;11)(q13;q34), del(13)(q11), add(16)(p11), der(16)t(13;16)(q31;q21) der(17)t(1;17)(?;p13), add(18)(q21)x2, del(19)(q11); carries simultaneous IGH-CCND1 and IGH-MYC rearrangements; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | KMS-12-PE |
|---|---|
| 商品货号： | TS259955 |
| <strong>Cell line </strong>： | KMS-12-PE |
| DSMZ no. ： | TS259955 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | multiple myeloma |
| Origin ： | established in 1987 from the pleural effusion of a 64-year-old woman with refractory, terminal multiple myeloma (Ig-non-producing) after combination chemotherapy; described in the literature to carry t(11:14)(q13:q32) rearrangement; sister cell line of KMS-12-BM (DSM ACC 551) |
| Reference(s) ： | 14799, 14817, 14818 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or occasionally in small clumps in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | optimal split ratio of 1:2 to 1:4 every 2-5 days; maintain at 0.5-1.0 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml; cells should be cultured in 24-well plates to maximum density at ca. 2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | maximum density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD34 -, CD38 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile (identical to that of sister cell line KMS-12-BM; ACC 551) |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy; 42(40-47)<2n>X,-X, +1, +7, -14, +16, +18, -22, der(1;16)(q10;p10), der(1)(1pter->q12::18q1?-q2?1::14q21.2->q32.32::11q.13->qter)qdp(1q12.1->q12::18q1?->q21), der(3)dup(3)(q26q29)t(3;21)(q29;q11.2), del(4)(q26), der(5)t(5;17)(p10;q10), der(6)t(1;6)(q32;q22), der(7)t(7;10)(p21;p13), der(8)ins(8;14)(q24;q32.3q32.3)x2, ider(9)(q10)t(9;11)(q24;q11), der(10;16)(q10;q10), der(11)t(11;14)(q13;q32), der(11)t(9;11)(q13;q34), del(13)(q11), add(16)(p11), der(16)t(13;16)(q31;q21) der(17)t(1;17)(?;p13), add(18)(q21)x2, del(19)(q11); carries simultaneous IGH-CCND1 and IGH-MYC rearrangements; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
