SUP-T11
SUP-T11
规格:
货期:
编号:TS259957
品牌:Testobio
| 产品名称： | SUP-T11 |
|---|---|
| 商品货号： | TS259957 |
| <strong>Cell line </strong>： | SUP-T11 |
| DSMZ no. ： | TS259957 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | T cell leukemia |
| Origin ： | derived from the bone marrow of a 74-year-old man with T-ALL at diagnosis; cell line was established in the presence of IGF-I under hypoxic conditions; cells were described in the literature to express high levels of TCL1A and TCL1B genes due to their juxtaposition from chromosome 14q32.1 to the regulatory element of the T-cell receptor C alpha/delta locus at chromosome 14q11 |
| Reference(s) ： | 14993, 15011, 15045, 14971 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, polymorph cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at ca. 0.2-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-45 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD20 -, CD34 -, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hypodiploid karyotype with 5% polyploidy; 38(36-39)<2n>XX, -Y, -10, -13, -15, -16, -17, -21, -22, del(X)(q11?q23), add(X)(q23), der(1)del(1)(p32)t(1;4)(q32;q32.2), der(1)t(1;7)(q32;q32), der(2)t(2;4)(p23;p15 or q35), der(3)t(1;3)(p33;q28), der(4)t(1;4)(p32;q32.2), dup(4)(p11p14), der(5)t(5;9)(p15;q11.2), der(6)t(1;6)(p3?2;p2?4), add(6)(q27), der(7)t(7;15)(q31.2;q21.2), add(8)(q24.33), der(8)t(8;16)(p12;p13), del(9)(q21q23), der(12)t(8;12)(q24;p13)t(12;16)(q24;23), t(14;14)(q11.2;q32.1), der(15)t(1;15)(q31;q26), der(19)t(19;21)(p13;q21), der(20)t(17;20)(p11;q13), der(21)t(12;21)(?q24;q12), add(22)(q13); resembles published karyotype; t(14;14) effects activation of TCL1 by juxtaposition with T-cell receptor A/D locus |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
SUP-T11
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SUP-T11 |
|---|---|
| 商品货号： | TS259957 |
| <strong>Cell line </strong>： | SUP-T11 |
| DSMZ no. ： | TS259957 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | T cell leukemia |
| Origin ： | derived from the bone marrow of a 74-year-old man with T-ALL at diagnosis; cell line was established in the presence of IGF-I under hypoxic conditions; cells were described in the literature to express high levels of TCL1A and TCL1B genes due to their juxtaposition from chromosome 14q32.1 to the regulatory element of the T-cell receptor C alpha/delta locus at chromosome 14q11 |
| Reference(s) ： | 14993, 15011, 15045, 14971 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, polymorph cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at ca. 0.2-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-45 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD20 -, CD34 -, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hypodiploid karyotype with 5% polyploidy; 38(36-39)<2n>XX, -Y, -10, -13, -15, -16, -17, -21, -22, del(X)(q11?q23), add(X)(q23), der(1)del(1)(p32)t(1;4)(q32;q32.2), der(1)t(1;7)(q32;q32), der(2)t(2;4)(p23;p15 or q35), der(3)t(1;3)(p33;q28), der(4)t(1;4)(p32;q32.2), dup(4)(p11p14), der(5)t(5;9)(p15;q11.2), der(6)t(1;6)(p3?2;p2?4), add(6)(q27), der(7)t(7;15)(q31.2;q21.2), add(8)(q24.33), der(8)t(8;16)(p12;p13), del(9)(q21q23), der(12)t(8;12)(q24;p13)t(12;16)(q24;23), t(14;14)(q11.2;q32.1), der(15)t(1;15)(q31;q26), der(19)t(19;21)(p13;q21), der(20)t(17;20)(p11;q13), der(21)t(12;21)(?q24;q12), add(22)(q13); resembles published karyotype; t(14;14) effects activation of TCL1 by juxtaposition with T-cell receptor A/D locus |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
