SCC-4
SCC-4
规格:
货期:
编号:TS259972
品牌:Testobio
| 产品名称： | SCC-4 |
|---|---|
| 商品货号： | TS259972 |
| <strong>Cell line </strong>： | SCC-4 |
| DSMZ no. ： | TS259972 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from the squamous cell carcinoma of the tongue from a 55-year-old man after radio- and chemotherapy in 1980 |
| Reference(s) ： | 14889 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 40 ng/ml hydrocortisone |
| Subculture ： | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum density at ca. 4 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded heteroploid karyotype with pentaploid and decaploid peaks; 100-117/202-266<5N/10N>XXYY/XXXXYYYY, add(Y)(q12), add(2)(q23), add(3)(q28), del(3)(p11), add(4)(p15), ins(4;?)(q32;??), incomplete |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
SCC-4
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SCC-4 |
|---|---|
| 商品货号： | TS259972 |
| <strong>Cell line </strong>： | SCC-4 |
| DSMZ no. ： | TS259972 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from the squamous cell carcinoma of the tongue from a 55-year-old man after radio- and chemotherapy in 1980 |
| Reference(s) ： | 14889 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 40 ng/ml hydrocortisone |
| Subculture ： | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum density at ca. 4 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded heteroploid karyotype with pentaploid and decaploid peaks; 100-117/202-266<5N/10N>XXYY/XXXXYYYY, add(Y)(q12), add(2)(q23), add(3)(q28), del(3)(p11), add(4)(p15), ins(4;?)(q32;??), incomplete |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
