SCC-25
SCC-25
规格:
货期:
编号:TS259975
品牌:Testobio
| 产品名称： | SCC-25 |
|---|---|
| 商品货号： | TS259975 |
| <strong>Cell line </strong>： | SCC-25 |
| DSMZ no. ： | TS259975 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from the biopsy of a squamous cell carcinoma of the tongue of a 70-year-old man |
| Reference(s) ： | 14889 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers; image; image |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hypertriploid karyotype with 4% polyploidy; 72-82<3n>XX, -Y, +4, -7, +9, +9, -13, -14, +15, -18, -19, -21, +10, -12 mar; add(1)(p31)x2, add(3)(?p11), add(4)(q11), i(5p), i(5q), der(8)t(1;8)(q25;p11)x1-2, del(9)(p11), i(9q), add(11)(q25), i(13q), add(14)(p11), add(15)(p11)x3-4, add(21)(q22) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
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朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | SCC-25 |
|---|---|
| 商品货号： | TS259975 |
| <strong>Cell line </strong>： | SCC-25 |
| DSMZ no. ： | TS259975 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from the biopsy of a squamous cell carcinoma of the tongue of a 70-year-old man |
| Reference(s) ： | 14889 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers; image; image |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hypertriploid karyotype with 4% polyploidy; 72-82<3n>XX, -Y, +4, -7, +9, +9, -13, -14, +15, -18, -19, -21, +10, -12 mar; add(1)(p31)x2, add(3)(?p11), add(4)(q11), i(5p), i(5q), der(8)t(1;8)(q25;p11)x1-2, del(9)(p11), i(9q), add(11)(q25), i(13q), add(14)(p11), add(15)(p11)x3-4, add(21)(q22) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
