| 产品名称: | FL-64 |
|---|---|
| 商品货号: | TS259977 |
| <strong>Cell line </strong>: | FL-64 |
| DSMZ no. : | TS259977 |
| Species : | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | hepatocellular tumor |
| Origin : | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat |
| Reference(s) : | 15145 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epitheloid cells growing adherently in monolayers |
| Medium : | 80% MEM (with Earles salts) + 20% h.i. FBS |
| Subculture : | seed out at ca. 2 x 104 cells/cm2; optimal split ratio of 1:2 to 1:3 every 5-8 days using trypsin/EDTA; cells grow slowly for about one week after thawing |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 60-100 hours |
| Harvest : | cell harvest of ca. 1 x 105 cells/cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Viruses : | PCR: SMRV - |
| 产品名称: | FL-64 |
|---|---|
| 商品货号: | TS259977 |
| <strong>Cell line </strong>: | FL-64 |
| DSMZ no. : | TS259977 |
| Species : | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | hepatocellular tumor |
| Origin : | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat |
| Reference(s) : | 15145 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epitheloid cells growing adherently in monolayers |
| Medium : | 80% MEM (with Earles salts) + 20% h.i. FBS |
| Subculture : | seed out at ca. 2 x 104 cells/cm2; optimal split ratio of 1:2 to 1:3 every 5-8 days using trypsin/EDTA; cells grow slowly for about one week after thawing |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 60-100 hours |
| Harvest : | cell harvest of ca. 1 x 105 cells/cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Viruses : | PCR: SMRV - |