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FL-64
FL-64
规格:
货期:
编号:TS259977
品牌:Testobio
产品名称: FL-64
商品货号: TS259977
<strong>Cell line </strong>: FL-64
DSMZ no. : TS259977
Species : confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding
Cell type : hepatocellular tumor
Origin : established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat
Reference(s) : 15145
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epitheloid cells growing adherently in monolayers
Medium : 80% MEM (with Earles salts) + 20% h.i. FBS
Subculture : seed out at ca. 2 x 104 cells/cm2; optimal split ratio of 1:2 to 1:3 every 5-8 days using trypsin/EDTA; cells grow slowly for about one week after thawing
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 60-100 hours
Harvest : cell harvest of ca. 1 x 105 cells/cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Viruses : PCR: SMRV -
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FL-64

  • 货号: TS259977
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: FL-64
商品货号: TS259977
<strong>Cell line </strong>: FL-64
DSMZ no. : TS259977
Species : confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding
Cell type : hepatocellular tumor
Origin : established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat
Reference(s) : 15145
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epitheloid cells growing adherently in monolayers
Medium : 80% MEM (with Earles salts) + 20% h.i. FBS
Subculture : seed out at ca. 2 x 104 cells/cm2; optimal split ratio of 1:2 to 1:3 every 5-8 days using trypsin/EDTA; cells grow slowly for about one week after thawing
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 60-100 hours
Harvest : cell harvest of ca. 1 x 105 cells/cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Viruses : PCR: SMRV -
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