CL-44
CL-44
规格:
货期:
编号:TS259980
品牌:Testobio
| 产品名称： | CL-44 |
|---|---|
| 商品货号： | TS259980 |
| <strong>Cell line </strong>： | CL-44 |
| DSMZ no. ： | TS259980 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatocellular tumor |
| Origin ： | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat |
| Reference(s) ： | 14614, 14658 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; optimal split ratio 1:3 to 1:5 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Viruses ： | PCR: SMRV - |
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朱静
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李晶晶
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周雪
13372109301
| 产品名称： | CL-44 |
|---|---|
| 商品货号： | TS259980 |
| <strong>Cell line </strong>： | CL-44 |
| DSMZ no. ： | TS259980 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatocellular tumor |
| Origin ： | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat |
| Reference(s) ： | 14614, 14658 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; optimal split ratio 1:3 to 1:5 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Viruses ： | PCR: SMRV - |
