HB-894
HB-894
规格:
货期:
编号:TS259998
品牌:Testobio
| 产品名称： | HB-894 |
|---|---|
| 商品货号： | TS259998 |
| <strong>Cell line </strong>： | HB-894 |
| DSMZ no. ： | TS259998 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from a spontaneously developed rhabdomyosarcoma of a Wistar AF/Han rat |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle-shaped cells growing as monolayer or in small clumps |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA for 10 min; after trypsination cells might remain in aggregates that are difficult to disperse and to count |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3 days |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Viruses ： | SMRV - |
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HB-894
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | HB-894 |
|---|---|
| 商品货号： | TS259998 |
| <strong>Cell line </strong>： | HB-894 |
| DSMZ no. ： | TS259998 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from a spontaneously developed rhabdomyosarcoma of a Wistar AF/Han rat |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle-shaped cells growing as monolayer or in small clumps |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA for 10 min; after trypsination cells might remain in aggregates that are difficult to disperse and to count |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3 days |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Viruses ： | SMRV - |
