SK-N-BE(2)
SK-N-BE(2)
规格:
货期:
编号:TS260000
品牌:Testobio
| 产品名称： | SK-N-BE(2) |
|---|---|
| 商品货号： | TS260000 |
| <strong>Cell line </strong>： | SK-N-BE(2) |
| DSMZ no. ： | TS260000 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | neuroblastoma |
| Origin ： | established in 1972 from the bone marrow biopsy of a 22-month-old boy with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy |
| Reference(s) ： | 14389, 14390, 14391 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% mixture of Hams F12 + MEM (with Earles salts)(at 1:1) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:6 - 1:10 every week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | maximum density at ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 12% polyploidy 43<2n>(42-49)X, -Y, +6, -9, -17, -18, der(1)t(1;2)(p13;p15), der(2)i(2)(p10)del(2)(p?13p24), der(3)t(3;17)(p21;q21), add(4)(q25), der(4)t(1;4)(q21;p15), add(6)(q25), add(8)(p12), der(10)add(10)(p13)add(10)(q21)(hsr), add(11)(p13), add(11)(q11), add(14)(q31), add(19)(q12); carries large hsr marker with genomic amplification of NMYC; resembles published karyotypes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SK-N-BE(2) |
|---|---|
| 商品货号： | TS260000 |
| <strong>Cell line </strong>： | SK-N-BE(2) |
| DSMZ no. ： | TS260000 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | neuroblastoma |
| Origin ： | established in 1972 from the bone marrow biopsy of a 22-month-old boy with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy |
| Reference(s) ： | 14389, 14390, 14391 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% mixture of Hams F12 + MEM (with Earles salts)(at 1:1) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:6 - 1:10 every week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | maximum density at ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 12% polyploidy 43<2n>(42-49)X, -Y, +6, -9, -17, -18, der(1)t(1;2)(p13;p15), der(2)i(2)(p10)del(2)(p?13p24), der(3)t(3;17)(p21;q21), add(4)(q25), der(4)t(1;4)(q21;p15), add(6)(q25), add(8)(p12), der(10)add(10)(p13)add(10)(q21)(hsr), add(11)(p13), add(11)(q11), add(14)(q31), add(19)(q12); carries large hsr marker with genomic amplification of NMYC; resembles published karyotypes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
