| 产品名称: | GUMBUS |
|---|---|
| 商品货号: | TS260004 |
| <strong>Cell line </strong>: | GUMBUS |
| DSMZ no. : | TS260004 |
| Species : | confirmed as human by species PCR |
| Cell type : | Burkitt lymphoma |
| Origin : | established from the liquor of a 28-year-old man with European Burkitt lymphoma at second relapse that became highly resistant to several chemotherapy regimens |
| Reference(s) : | 14404 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | small round cells growing in clumps in suspension; image; image; image |
| Medium : | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture : | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5 x 106 cells/ml; optimal split ratio 1:2 to 1:3 every 2-3 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | maximum density at ca. 2.0 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics : | human near diploid karyotype with 4% polyploidy 46(44-46)<2n>,XY, del(3)(p11p21.2), del(6)(q14q24), t(8;14)(q24;q32); carries t(8;14) effecting rearrangement of MYC with IGH; resembles published karyotype |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| 产品名称: | GUMBUS |
|---|---|
| 商品货号: | TS260004 |
| <strong>Cell line </strong>: | GUMBUS |
| DSMZ no. : | TS260004 |
| Species : | confirmed as human by species PCR |
| Cell type : | Burkitt lymphoma |
| Origin : | established from the liquor of a 28-year-old man with European Burkitt lymphoma at second relapse that became highly resistant to several chemotherapy regimens |
| Reference(s) : | 14404 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | small round cells growing in clumps in suspension; image; image; image |
| Medium : | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture : | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5 x 106 cells/ml; optimal split ratio 1:2 to 1:3 every 2-3 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | maximum density at ca. 2.0 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics : | human near diploid karyotype with 4% polyploidy 46(44-46)<2n>,XY, del(3)(p11p21.2), del(6)(q14q24), t(8;14)(q24;q32); carries t(8;14) effecting rearrangement of MYC with IGH; resembles published karyotype |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |