293T
293T
规格:
货期:
编号:TS260016
品牌:Testobio
| 产品名称： | 293T |
|---|---|
| 商品货号： | TS260016 |
| <strong>Cell line </strong>： | 293T |
| DSMZ no. ： | TS260016 |
| Species ： | confirmed originally as human by species PCR and later by STR fingerprinting |
| Cell type ： | embryonal kidney |
| Origin ： | highly transfectable derivative of the human primary embryonal kidney cell line 293 (ACC 305) carrying a plasmid containing the temperature sensitive mutant of SV-40 large T-antigen (tsA1609neo); cells originally referred to as 293tsA1609neo; cells were described in the literature to be Geniticin (Neomycin, G418) resistant and to express SV-40 large T at 33°C, but not at 40°C; classified as risk group 1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14893, 14495, 14839 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | oval to fibroblastoid cells growing adherently as monolayer; image; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-30 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as 293 (ACC 305) by fluorescent nonaplex PCR of short tandem repeat markers |
| Cytogenetics ： | human flat-moded near-triploid karyotype; 61-73<3n>XXX, add(1)(p32), add(1)(q44)x1-2, der(4;10)(q10;q10), del(8)(p12), t(9;9)(p24;q21), der(13)t(6;13)(q21;q32), der(14)t(13;14)(q32;q32), del(17)(p11), der(22)t(17;22)(q21;q22) |
| Molec. Genetics ： | presence of large T-antigene (SV40) was confirmed by genomic PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
293T
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | 293T |
|---|---|
| 商品货号： | TS260016 |
| <strong>Cell line </strong>： | 293T |
| DSMZ no. ： | TS260016 |
| Species ： | confirmed originally as human by species PCR and later by STR fingerprinting |
| Cell type ： | embryonal kidney |
| Origin ： | highly transfectable derivative of the human primary embryonal kidney cell line 293 (ACC 305) carrying a plasmid containing the temperature sensitive mutant of SV-40 large T-antigen (tsA1609neo); cells originally referred to as 293tsA1609neo; cells were described in the literature to be Geniticin (Neomycin, G418) resistant and to express SV-40 large T at 33°C, but not at 40°C; classified as risk group 1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14893, 14495, 14839 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | oval to fibroblastoid cells growing adherently as monolayer; image; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-30 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as 293 (ACC 305) by fluorescent nonaplex PCR of short tandem repeat markers |
| Cytogenetics ： | human flat-moded near-triploid karyotype; 61-73<3n>XXX, add(1)(p32), add(1)(q44)x1-2, der(4;10)(q10;q10), del(8)(p12), t(9;9)(p24;q21), der(13)t(6;13)(q21;q32), der(14)t(13;14)(q32;q32), del(17)(p11), der(22)t(17;22)(q21;q22) |
| Molec. Genetics ： | presence of large T-antigene (SV40) was confirmed by genomic PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
