U-2973
U-2973
规格:
货期:
编号:TS260019
品牌:Testobio
| 产品名称： | U-2973 |
|---|---|
| 商品货号： | TS260019 |
| <strong>Cell line </strong>： | U-2973 |
| DSMZ no. ： | TS260019 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2006 from the peripheral blood of a 42-year-old Caucasian man with mature B-cell leukemia; described in the literature to carry t(14;18)(q32;q21) with IGH-BCL2 and biallelic MYC rearrangement with non-IG partner |
| Reference(s) ： | 14402 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-2.0 x106 cells/ml, optimal split ratio of 1:3 to 1:6 every 3-5 days; viability may drop below 50% within the first 3-4 days after thawing; we recommend to start culture with a cell concentration of 1.0 x 106 cells/ml and to cultivate the cells initially in 24-well-plates in the presence of 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; 47(44-48)XY, +12, del(6)(q24.2q26), t(8;12)(q24;p12)x2, t(14;18)(q32;q21), der(20)t(18;20;q21;q13); t(14;18) effects rearrangement of IGH and BCL2; t(8;12) effects non-IG, MYC rearrangement; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | U-2973 |
|---|---|
| 商品货号： | TS260019 |
| <strong>Cell line </strong>： | U-2973 |
| DSMZ no. ： | TS260019 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2006 from the peripheral blood of a 42-year-old Caucasian man with mature B-cell leukemia; described in the literature to carry t(14;18)(q32;q21) with IGH-BCL2 and biallelic MYC rearrangement with non-IG partner |
| Reference(s) ： | 14402 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-2.0 x106 cells/ml, optimal split ratio of 1:3 to 1:6 every 3-5 days; viability may drop below 50% within the first 3-4 days after thawing; we recommend to start culture with a cell concentration of 1.0 x 106 cells/ml and to cultivate the cells initially in 24-well-plates in the presence of 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; 47(44-48)XY, +12, del(6)(q24.2q26), t(8;12)(q24;p12)x2, t(14;18)(q32;q21), der(20)t(18;20;q21;q13); t(14;18) effects rearrangement of IGH and BCL2; t(8;12) effects non-IG, MYC rearrangement; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
