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PK-15
PK-15
规格:
货期:
编号:TS260021
品牌:Testobio
产品名称: PK-15
商品货号: TS260021
<strong>Cell line </strong>: PK-15
DSMZ no. : TS260021
Species : originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding
Cell type : kidney
Origin : derived by single-cell cloning of the parent cell line PK-2a in 1957, which was established in 1955 from the kidney of an adult pig; cells were shown to carry an endogenous C-type retrovirus and to be susceptible to various viruses, e.g. viruses causing Hog cholera, African swine fever, and foot and mouth disease
Reference(s) : 15017, 14360, 14853
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epitheloid cells growing adherently as monolayer; image; image; image
Medium : 90% Dulbecco´s MEM + 10% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:10 every 3 days using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 25 hours
Harvest : maximum densitiy of ca. 8-10 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Viruses : SMRV -
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PK-15

  • 货号: TS260021
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: PK-15
商品货号: TS260021
<strong>Cell line </strong>: PK-15
DSMZ no. : TS260021
Species : originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding
Cell type : kidney
Origin : derived by single-cell cloning of the parent cell line PK-2a in 1957, which was established in 1955 from the kidney of an adult pig; cells were shown to carry an endogenous C-type retrovirus and to be susceptible to various viruses, e.g. viruses causing Hog cholera, African swine fever, and foot and mouth disease
Reference(s) : 15017, 14360, 14853
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epitheloid cells growing adherently as monolayer; image; image; image
Medium : 90% Dulbecco´s MEM + 10% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:10 every 3 days using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 25 hours
Harvest : maximum densitiy of ca. 8-10 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Viruses : SMRV -
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