| 产品名称: | PK-15 |
|---|---|
| 商品货号: | TS260021 |
| <strong>Cell line </strong>: | PK-15 |
| DSMZ no. : | TS260021 |
| Species : | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type : | kidney |
| Origin : | derived by single-cell cloning of the parent cell line PK-2a in 1957, which was established in 1955 from the kidney of an adult pig; cells were shown to carry an endogenous C-type retrovirus and to be susceptible to various viruses, e.g. viruses causing Hog cholera, African swine fever, and foot and mouth disease |
| Reference(s) : | 15017, 14360, 14853 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epitheloid cells growing adherently as monolayer; image; image; image |
| Medium : | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:10 every 3 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 25 hours |
| Harvest : | maximum densitiy of ca. 8-10 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Viruses : | SMRV - |
| 产品名称: | PK-15 |
|---|---|
| 商品货号: | TS260021 |
| <strong>Cell line </strong>: | PK-15 |
| DSMZ no. : | TS260021 |
| Species : | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type : | kidney |
| Origin : | derived by single-cell cloning of the parent cell line PK-2a in 1957, which was established in 1955 from the kidney of an adult pig; cells were shown to carry an endogenous C-type retrovirus and to be susceptible to various viruses, e.g. viruses causing Hog cholera, African swine fever, and foot and mouth disease |
| Reference(s) : | 15017, 14360, 14853 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epitheloid cells growing adherently as monolayer; image; image; image |
| Medium : | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:10 every 3 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 25 hours |
| Harvest : | maximum densitiy of ca. 8-10 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Viruses : | SMRV - |