SH-2
SH-2
规格:
货期:
编号:TS260028
品牌:Testobio
| 产品名称： | SH-2 |
|---|---|
| 商品货号： | TS260028 |
| <strong>Cell line </strong>： | SH-2 |
| DSMZ no. ： | TS260028 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 2005 from the bone marrow of a 35-year-old Chinese man with AML-M2 after idarubicin/cysteine arabinoside treatment; cells were described in the literature to express natural killer cell-related antigens in addition to myeloid ones and to exhibit TP53 gene alteration; cells are tumorigenic in nude and SCID mice |
| Reference(s) ： | 14865 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and partially in clumps in suspension; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; we recommend to start culture in a 12- or 24-well plate; optimal split ratio of 1:2 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | maximum densitiy of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD56 +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy; 57(54-58)<2n>X, -Y, +1, +2, +3, +4, +6, -11, +12, +13, +15, +18, +19, +22, +2mar, del(1)(q31.2q42)x2, der(4)t(1;4)(p22;q32), der(16)t(16;17)(q24;q12); related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
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李晶晶
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周雪
13372109301
| 产品名称： | SH-2 |
|---|---|
| 商品货号： | TS260028 |
| <strong>Cell line </strong>： | SH-2 |
| DSMZ no. ： | TS260028 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 2005 from the bone marrow of a 35-year-old Chinese man with AML-M2 after idarubicin/cysteine arabinoside treatment; cells were described in the literature to express natural killer cell-related antigens in addition to myeloid ones and to exhibit TP53 gene alteration; cells are tumorigenic in nude and SCID mice |
| Reference(s) ： | 14865 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and partially in clumps in suspension; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; we recommend to start culture in a 12- or 24-well plate; optimal split ratio of 1:2 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | maximum densitiy of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD56 +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy; 57(54-58)<2n>X, -Y, +1, +2, +3, +4, +6, -11, +12, +13, +15, +18, +19, +22, +2mar, del(1)(q31.2q42)x2, der(4)t(1;4)(p22;q32), der(16)t(16;17)(q24;q12); related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
