SHI-1
SHI-1
规格:
货期:
编号:TS260031
品牌:Testobio
| 产品名称： | SHI-1 |
|---|---|
| 商品货号： | TS260031 |
| <strong>Cell line </strong>： | SHI-1 |
| DSMZ no. ： | TS260031 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myelocytic leukemia |
| Origin ： | established in 2002 from the bone marrow of a 37-year-old male with relapsed acute monocytic leukemia (AML-M5b); described in the literature to carry t(6;11)(q27;q23) with KMT2A-AFDN (MLL-MLLT4; MLL-AF6) rearrangement and a TP53 point mutation |
| Reference(s) ： | 14429 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days; maintain at 0.3-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum densitiy of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, CD41 +, CD235a -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 12% polyploidy; 46(44-46)<2n>XY, -18, +mar, t(6;11)(q27;q23), add(8)(p23), del(10)(p11), del(17)(p12); carries t(6;11) effecting rearrangement of KMT2A (MLL) with AFDN (MLLT4; AF6); matches published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFDN (MLL-MLLT4; MLL-AF6) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
SHI-1
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SHI-1 |
|---|---|
| 商品货号： | TS260031 |
| <strong>Cell line </strong>： | SHI-1 |
| DSMZ no. ： | TS260031 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myelocytic leukemia |
| Origin ： | established in 2002 from the bone marrow of a 37-year-old male with relapsed acute monocytic leukemia (AML-M5b); described in the literature to carry t(6;11)(q27;q23) with KMT2A-AFDN (MLL-MLLT4; MLL-AF6) rearrangement and a TP53 point mutation |
| Reference(s) ： | 14429 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days; maintain at 0.3-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum densitiy of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, CD41 +, CD235a -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 12% polyploidy; 46(44-46)<2n>XY, -18, +mar, t(6;11)(q27;q23), add(8)(p23), del(10)(p11), del(17)(p12); carries t(6;11) effecting rearrangement of KMT2A (MLL) with AFDN (MLLT4; AF6); matches published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFDN (MLL-MLLT4; MLL-AF6) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
