HCEC-12
HCEC-12
规格:
货期:
编号:TS260037
品牌:Testobio
| 产品名称： | HCEC-12 |
|---|---|
| 商品货号： | TS260037 |
| <strong>Cell line </strong>： | HCEC-12 |
| DSMZ no. ： | TS260037 |
| Species ： | human (Homo sapiens) |
| Cell type ： | corneal endothelium |
| Origin ： | established in 1996 from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen |
| Reference(s) ： | 14376 |
| Biosafety level ： | 1, GMO-S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers; image; image; image |
| Medium ： | 95% mixture of Hams F12 + Medium 199 (at 1:1) + 5% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; flasks must be coated with 10 µg/ml laminin and 10 mg/ml chondroitin sulfate; split confluent culture 1:2 to 1:4 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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李晶晶
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周雪
13372109301
| 产品名称： | HCEC-12 |
|---|---|
| 商品货号： | TS260037 |
| <strong>Cell line </strong>： | HCEC-12 |
| DSMZ no. ： | TS260037 |
| Species ： | human (Homo sapiens) |
| Cell type ： | corneal endothelium |
| Origin ： | established in 1996 from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen |
| Reference(s) ： | 14376 |
| Biosafety level ： | 1, GMO-S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers; image; image; image |
| Medium ： | 95% mixture of Hams F12 + Medium 199 (at 1:1) + 5% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; flasks must be coated with 10 µg/ml laminin and 10 mg/ml chondroitin sulfate; split confluent culture 1:2 to 1:4 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
