WILL-1
WILL-1
规格:
货期:
编号:TS260039
品牌:Testobio
| 产品名称： | WILL-1 |
|---|---|
| 商品货号： | TS260039 |
| <strong>Cell line </strong>： | WILL-1 |
| DSMZ no. ： | TS260039 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2007 from the bone marrow mononuclear cells of an 82-year-old Japanese man with diffuse large B-cell lymphoma in the leukemic phase |
| Reference(s) ： | 14973, 15029 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round or teardrop-shaped cells growing singly or in clumps in suspension; image; image; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at 0.7-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-5 days; maintain at 0.3-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours; but 50-70 hours after recovery from liquid nitrogen |
| Harvest ： | maximum densitiy of ca. 3.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +,CD80 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hypodiploid/hyperdiploid karyotype; 45(41-46)<2n>X, -Y, add(1)(q24), der(2)t(1;2)(q12;q3?7)inv(1)(q2?4q4?1), i(4q), der(6)t(6;8)(q27;q24), t(8;14)(q24;q32), der(17)t(X;17)(q23;p13), i(21q); sdl with idem, 57(50-58)<2n>X, -Y, +5, +6, +7, +8, +9, +11, +13, +der(14), +20, +21; carries t(8;14) effecting IGH/MYC rearrangement confirmed by FISH; non-IGH-MYC rearrangement present in der(6); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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联系我们CONTACT US
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0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | WILL-1 |
|---|---|
| 商品货号： | TS260039 |
| <strong>Cell line </strong>： | WILL-1 |
| DSMZ no. ： | TS260039 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2007 from the bone marrow mononuclear cells of an 82-year-old Japanese man with diffuse large B-cell lymphoma in the leukemic phase |
| Reference(s) ： | 14973, 15029 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round or teardrop-shaped cells growing singly or in clumps in suspension; image; image; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at 0.7-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-5 days; maintain at 0.3-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours; but 50-70 hours after recovery from liquid nitrogen |
| Harvest ： | maximum densitiy of ca. 3.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +,CD80 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hypodiploid/hyperdiploid karyotype; 45(41-46)<2n>X, -Y, add(1)(q24), der(2)t(1;2)(q12;q3?7)inv(1)(q2?4q4?1), i(4q), der(6)t(6;8)(q27;q24), t(8;14)(q24;q32), der(17)t(X;17)(q23;p13), i(21q); sdl with idem, 57(50-58)<2n>X, -Y, +5, +6, +7, +8, +9, +11, +13, +der(14), +20, +21; carries t(8;14) effecting IGH/MYC rearrangement confirmed by FISH; non-IGH-MYC rearrangement present in der(6); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
