WILL-2
WILL-2
规格:
货期:
编号:TS260043
品牌:Testobio
| 产品名称： | WILL-2 |
|---|---|
| 商品货号： | TS260043 |
| <strong>Cell line </strong>： | WILL-2 |
| DSMZ no. ： | TS260043 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2008 post-mortem from the ascites of a 63-year-old Japanese woman with diffuse large B-cell lymphoma after relapse |
| Reference(s) ： | 14974 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small and mostly single round cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in a 24-well-plate; cells may grow slowly during the first days after thawing, but recover soon and grow fast in log-phase; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at 1-2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36-48 hours |
| Harvest ： | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD38 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human near-diploid karyotype; 46(43-47)<2n>XX, del(X)(q23), dup(1)(q21q32), ins(1;X)(q21;q27q23), t(4;17)(p15;q12). t(5;9)(q14;p21), t(8;22)(q24;q11), t(14;18)(q32;q21); carries t(8;22) rearrangement effecting IGL-MYC, and t(14;18) rearrangements effecting IGH-BCL2; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | WILL-2 |
|---|---|
| 商品货号： | TS260043 |
| <strong>Cell line </strong>： | WILL-2 |
| DSMZ no. ： | TS260043 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2008 post-mortem from the ascites of a 63-year-old Japanese woman with diffuse large B-cell lymphoma after relapse |
| Reference(s) ： | 14974 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small and mostly single round cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in a 24-well-plate; cells may grow slowly during the first days after thawing, but recover soon and grow fast in log-phase; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at 1-2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36-48 hours |
| Harvest ： | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD38 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human near-diploid karyotype; 46(43-47)<2n>XX, del(X)(q23), dup(1)(q21q32), ins(1;X)(q21;q27q23), t(4;17)(p15;q12). t(5;9)(q14;p21), t(8;22)(q24;q11), t(14;18)(q32;q21); carries t(8;22) rearrangement effecting IGL-MYC, and t(14;18) rearrangements effecting IGH-BCL2; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
