GI-ME-N
GI-ME-N
规格:
货期:
编号:TS260049
品牌:Testobio
| 产品名称： | GI-ME-N |
|---|---|
| 商品货号： | TS260049 |
| <strong>Cell line </strong>： | GI-ME-N |
| DSMZ no. ： | TS260049 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established from a bone marrow metastasis of a 42-month-old girl with stage IV neuroblastoma after 6 months of chemotherapy |
| Reference(s) ： | 14466, 18136, 15180, 15196 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorph cells growing adherently in monolayers; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | GI-ME-N |
|---|---|
| 商品货号： | TS260049 |
| <strong>Cell line </strong>： | GI-ME-N |
| DSMZ no. ： | TS260049 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established from a bone marrow metastasis of a 42-month-old girl with stage IV neuroblastoma after 6 months of chemotherapy |
| Reference(s) ： | 14466, 18136, 15180, 15196 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorph cells growing adherently in monolayers; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
