NBL-S
NBL-S
规格:
货期:
编号:TS260055
品牌:Testobio
| 产品名称： | NBL-S |
|---|---|
| 商品货号： | TS260055 |
| <strong>Cell line </strong>： | NBL-S |
| DSMZ no. ： | TS260055 |
| Species ： | human by species-PCR and DNA fingerprint |
| Cell type ： | neuroblastoma |
| Origin ： | established from the primary adrenal tumor resected prior to treatment from a 3-year-old boy with rapidly progressing Stage III neuroblastoma in 1989; described in the literature to lack MYCN (NMYC, N-myc) amplification and chromosome 1p deletions, but to show prolonged N-myc protein half life |
| Reference(s) ： | 14435, 14571 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorph cells growing adherently in monolayers; image; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:6 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | cell harvest of ca. 60 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | NBL-S |
|---|---|
| 商品货号： | TS260055 |
| <strong>Cell line </strong>： | NBL-S |
| DSMZ no. ： | TS260055 |
| Species ： | human by species-PCR and DNA fingerprint |
| Cell type ： | neuroblastoma |
| Origin ： | established from the primary adrenal tumor resected prior to treatment from a 3-year-old boy with rapidly progressing Stage III neuroblastoma in 1989; described in the literature to lack MYCN (NMYC, N-myc) amplification and chromosome 1p deletions, but to show prolonged N-myc protein half life |
| Reference(s) ： | 14435, 14571 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorph cells growing adherently in monolayers; image; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:6 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | cell harvest of ca. 60 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
