LAN-1
LAN-1
规格:
货期:
编号:TS260056
品牌:Testobio
| 产品名称： | LAN-1 |
|---|---|
| 商品货号： | TS260056 |
| <strong>Cell line </strong>： | LAN-1 |
| DSMZ no. ： | TS260056 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established in 1977 from a bone marrow metastasis of a 2-year-old boy with stage IV neuroblastoma |
| Reference(s) ： | 14949 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells are growing adherently and in clumps in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS or 80-90% MEM (with Earles salts) + 10-20% h.i. FBS + non-essential amino acids + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2 flask; split confluent culture 1:2 every 3-4 days using trypsin/EDTA (cells can also be detached by tapping the flask or by pipetting); viability can decrease shortly to 50-70% after seeding |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
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朱静
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李晶晶
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周雪
13372109301
| 产品名称： | LAN-1 |
|---|---|
| 商品货号： | TS260056 |
| <strong>Cell line </strong>： | LAN-1 |
| DSMZ no. ： | TS260056 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established in 1977 from a bone marrow metastasis of a 2-year-old boy with stage IV neuroblastoma |
| Reference(s) ： | 14949 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells are growing adherently and in clumps in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS or 80-90% MEM (with Earles salts) + 10-20% h.i. FBS + non-essential amino acids + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2 flask; split confluent culture 1:2 every 3-4 days using trypsin/EDTA (cells can also be detached by tapping the flask or by pipetting); viability can decrease shortly to 50-70% after seeding |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
