UPCI-SCC-099
UPCI-SCC-099
规格:
货期:
编号:TS260069
品牌:Testobio
| 产品名称： | UPCI-SCC-099 |
|---|---|
| 商品货号： | TS260069 |
| <strong>Cell line </strong>： | UPCI-SCC-099 |
| DSMZ no. ： | TS260069 |
| Species ： | human (Homo sapiens) |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from an oral squamous cell carcinoma of a 52-year-old Caucasian man; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 14735, 15061, 15176 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer; image; image |
| Medium ： | 80-90% MEM (with Earles salts) + 10-20% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA for more than 10 minutes |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
UPCI-SCC-099
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | UPCI-SCC-099 |
|---|---|
| 商品货号： | TS260069 |
| <strong>Cell line </strong>： | UPCI-SCC-099 |
| DSMZ no. ： | TS260069 |
| Species ： | human (Homo sapiens) |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from an oral squamous cell carcinoma of a 52-year-old Caucasian man; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 14735, 15061, 15176 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer; image; image |
| Medium ： | 80-90% MEM (with Earles salts) + 10-20% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA for more than 10 minutes |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
