UPCI-SCC-090
UPCI-SCC-090
规格:
货期:
编号:TS260083
品牌:Testobio
| 产品名称： | UPCI-SCC-090 |
|---|---|
| 商品货号： | TS260083 |
| <strong>Cell line </strong>： | UPCI-SCC-090 |
| DSMZ no. ： | TS260083 |
| Species ： | human (Homo sapiens) |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from an oral squamous cell carcinoma of a 44-year-old Caucasian man; described in the literature to carry 100-150 copies of integrated human papilloma virus type 16 (HPV-16); the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 14735, 15061, 14530, 18143 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cells are infected with human papilloma viruses (HPV) as detected by PCR. HPV sequences were also amplified applying PCR analysis on DNase I digested supernatant to detect DNase I protected active viruses. A transmission of HPV on handling the cells cannot be ruled out. The cell line was thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 every 6-10 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 120-150 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | UPCI-SCC-090 |
|---|---|
| 商品货号： | TS260083 |
| <strong>Cell line </strong>： | UPCI-SCC-090 |
| DSMZ no. ： | TS260083 |
| Species ： | human (Homo sapiens) |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from an oral squamous cell carcinoma of a 44-year-old Caucasian man; described in the literature to carry 100-150 copies of integrated human papilloma virus type 16 (HPV-16); the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 14735, 15061, 14530, 18143 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cells are infected with human papilloma viruses (HPV) as detected by PCR. HPV sequences were also amplified applying PCR analysis on DNase I digested supernatant to detect DNase I protected active viruses. A transmission of HPV on handling the cells cannot be ruled out. The cell line was thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 every 6-10 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 120-150 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/-II -, MLV - |
