LAN-2
LAN-2
规格:
货期:
编号:TS260084
品牌:Testobio
| 产品名称： | LAN-2 |
|---|---|
| 商品货号： | TS260084 |
| <strong>Cell line </strong>： | LAN-2 |
| DSMZ no. ： | TS260084 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established from the primary tumor of a 3-year-old girl with neuroblastoma (clinical Stage IV) |
| Reference(s) ： | 14949 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent flat cells with processes accompanied by loosely adherent round cells |
| Medium ： | 40% RPMI 1640 + 40% MEM (with Earles salts) + 20% FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; (cells might grow slowly after thawing) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 55 x 106 cells/175 cm2 |
| Storage ： | frozen with 35% RPMI 1640, 35% MEM (with Earles salts), 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | LAN-2 |
|---|---|
| 商品货号： | TS260084 |
| <strong>Cell line </strong>： | LAN-2 |
| DSMZ no. ： | TS260084 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established from the primary tumor of a 3-year-old girl with neuroblastoma (clinical Stage IV) |
| Reference(s) ： | 14949 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent flat cells with processes accompanied by loosely adherent round cells |
| Medium ： | 40% RPMI 1640 + 40% MEM (with Earles salts) + 20% FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; (cells might grow slowly after thawing) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 55 x 106 cells/175 cm2 |
| Storage ： | frozen with 35% RPMI 1640, 35% MEM (with Earles salts), 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
