LAN-6
LAN-6
规格:
货期:
编号:TS260088
品牌:Testobio
| 产品名称： | LAN-6 |
|---|---|
| 商品货号： | TS260088 |
| <strong>Cell line </strong>： | LAN-6 |
| DSMZ no. ： | TS260088 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established from a bone marrow metastasis of a 6-year-old boy with neuroblastoma (clinical Stage IV) |
| Reference(s) ： | 15481, 15482 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | loosely adherent, epithelioid cells growing as monolayers; image; image; image |
| Medium ： | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2 flask; cells grow rather slowly; split confluent culture 1:2 once a week using trypsin/EDTA (cells are only slightly adherent and can also be detached by tapping the flask or pipetting, but trypsin/EDTA is necessary to prepare single cells) |
| Incubation ： | at 37 °C with 5% to 10% CO2 |
| Doubling time ： | ca. one week |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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朱静
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周雪
13372109301
| 产品名称： | LAN-6 |
|---|---|
| 商品货号： | TS260088 |
| <strong>Cell line </strong>： | LAN-6 |
| DSMZ no. ： | TS260088 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established from a bone marrow metastasis of a 6-year-old boy with neuroblastoma (clinical Stage IV) |
| Reference(s) ： | 15481, 15482 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | loosely adherent, epithelioid cells growing as monolayers; image; image; image |
| Medium ： | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2 flask; cells grow rather slowly; split confluent culture 1:2 once a week using trypsin/EDTA (cells are only slightly adherent and can also be detached by tapping the flask or pipetting, but trypsin/EDTA is necessary to prepare single cells) |
| Incubation ： | at 37 °C with 5% to 10% CO2 |
| Doubling time ： | ca. one week |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
