NGP
NGP
规格:
货期:
编号:TS260090
品牌:Testobio
| 产品名称： | NGP |
|---|---|
| 商品货号： | TS260090 |
| <strong>Cell line </strong>： | NGP |
| DSMZ no. ： | TS260090 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established 1974 from lung metastases obtained at post mortem from a 2 1/2-year-old boy with neuroblastoma |
| Reference(s) ： | 14407, 15137 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers and in clumps; image; image |
| Medium ： | 90% Dulbecco´s MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 3-4 x 106 cells/80 cm2 flask; cells take about three days to become adherent; split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 40-50 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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0574-87917803
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testobio@163.com
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | NGP |
|---|---|
| 商品货号： | TS260090 |
| <strong>Cell line </strong>： | NGP |
| DSMZ no. ： | TS260090 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established 1974 from lung metastases obtained at post mortem from a 2 1/2-year-old boy with neuroblastoma |
| Reference(s) ： | 14407, 15137 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers and in clumps; image; image |
| Medium ： | 90% Dulbecco´s MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 3-4 x 106 cells/80 cm2 flask; cells take about three days to become adherent; split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 40-50 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
