BC-3
BC-3
规格:
货期:
编号:TS260103
品牌:Testobio
| 产品名称： | BC-3 |
|---|---|
| 商品货号： | TS260103 |
| <strong>Cell line </strong>： | BC-3 |
| DSMZ no. ： | TS260103 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1995 from the pleural effusion of a 85-year-old man with B-NHL (primary effusion lymphoma, PEL) not infected with HIV; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15851, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in clusters in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximum densitiy of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, smIgG -, smIgM -, smkappa -, smlambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat moded hyperdiploid karyotype; 47(43-48)<2n>X, -Y, +7, +8, der(1)t(1;16)(q44;q13), del(7)(p21.1p21.2), t(8;15)(p21;q26), del(13)(q13), der(14)t(12;14)(q11;p11),t(16;22)(q13;q12), der(19)t(19;19)(p13.1;q13.3), del(20)(p11.2p11.3); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | BC-3 |
|---|---|
| 商品货号： | TS260103 |
| <strong>Cell line </strong>： | BC-3 |
| DSMZ no. ： | TS260103 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1995 from the pleural effusion of a 85-year-old man with B-NHL (primary effusion lymphoma, PEL) not infected with HIV; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15851, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in clusters in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximum densitiy of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, smIgG -, smIgM -, smkappa -, smlambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat moded hyperdiploid karyotype; 47(43-48)<2n>X, -Y, +7, +8, der(1)t(1;16)(q44;q13), del(7)(p21.1p21.2), t(8;15)(p21;q26), del(13)(q13), der(14)t(12;14)(q11;p11),t(16;22)(q13;q12), der(19)t(19;19)(p13.1;q13.3), del(20)(p11.2p11.3); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
