KASUMI-6
KASUMI-6
规格:
货期:
编号:TS260109
品牌:Testobio
| 产品名称： | KASUMI-6 |
|---|---|
| 商品货号： | TS260109 |
| <strong>Cell line </strong>： | KASUMI-6 |
| DSMZ no. ： | TS260109 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1999 from peripheral blood of a 68-year-old Japanese man with acute myeloid leukemia (AML-M2) after relapse; cells are described in the literature to carry a dominant-negative CEBPA (C/EBPA) mutation |
| Reference(s) ： | 15974 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS (optional + 10 ng/ml GM-CSF) |
| Subculture ： | cells are difficult to culture; seed out at ca. 0.5 x 106 cells/ml, maintain at 0.4-1.0 x 106 cells/ml (do not exceed these limits), split saturated culture 1:2 every 3 days; upon thawing viability drop to about 50% and may need one week to recover; accumulation of cell debris may resemble a bacterial infection; it is recommended to culture the cells in 24-well-plates; cells were described to be constitutively cytokine-dependent, however, we found that the cells are no more cytokine dependent. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD14 -, CD19 -, CD33 +, CD34 -, cyCD68 -, HLA-DR + |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype; 47-51<2n>XY, +5, +8, +10, +13, +22, inv(5)(p11q32), add(6)(p22.1), add(8)(p23)x1-2, der(12;17)(q10;q10), der(13)t(13;13)(p12;q31), dup(19)(q11q13); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | KASUMI-6 |
|---|---|
| 商品货号： | TS260109 |
| <strong>Cell line </strong>： | KASUMI-6 |
| DSMZ no. ： | TS260109 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1999 from peripheral blood of a 68-year-old Japanese man with acute myeloid leukemia (AML-M2) after relapse; cells are described in the literature to carry a dominant-negative CEBPA (C/EBPA) mutation |
| Reference(s) ： | 15974 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS (optional + 10 ng/ml GM-CSF) |
| Subculture ： | cells are difficult to culture; seed out at ca. 0.5 x 106 cells/ml, maintain at 0.4-1.0 x 106 cells/ml (do not exceed these limits), split saturated culture 1:2 every 3 days; upon thawing viability drop to about 50% and may need one week to recover; accumulation of cell debris may resemble a bacterial infection; it is recommended to culture the cells in 24-well-plates; cells were described to be constitutively cytokine-dependent, however, we found that the cells are no more cytokine dependent. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD14 -, CD19 -, CD33 +, CD34 -, cyCD68 -, HLA-DR + |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype; 47-51<2n>XY, +5, +8, +10, +13, +22, inv(5)(p11q32), add(6)(p22.1), add(8)(p23)x1-2, der(12;17)(q10;q10), der(13)t(13;13)(p12;q31), dup(19)(q11q13); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
