BCBL-1
BCBL-1
规格:
货期:
编号:TS260111
品牌:Testobio
| 产品名称： | BCBL-1 |
|---|---|
| 商品货号： | TS260111 |
| <strong>Cell line </strong>： | BCBL-1 |
| DSMZ no. ： | TS260111 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peritoneal effusion of a 40-year-old man (HIV+) with B-NHL (primary effusion lymphoma, PEL); cells were shown to carry the human herpesvirus type 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15854, 14483, 15200, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round/multiformed cells growing singly in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; immediately after thawing, viability might be rather low (60-70%) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum densitiy of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertetraploid karyotype with near-diploid and near-octaploid sidelines; 91-100<4n>XX, -Y, -Y, +2, +2, +6, +7, +7, +8, -9, -10, -11, +12, -13, -13, +15, -16, -22, -22, +2, -5mar, dup(1)(q12q44), del(1)(p11)dup(1)(q12q44), del(2)(p11)x1-2, der(2)t(2;5)(q35;?q32)t(5;8)(?q35;q22), der(4)t(4;8)(q24;q24), t(6;17)(p23;q21), del(9)(q12q21), der(11)t(11;15)(q23;q21), der(11)t(11;15)(q25;q25), der(13)t(13;13)(p12;q21)x1-2, der(14)t(4;14)(q31;q32)x2, der(14)t(14;22)(p12;q12)t(4;14)(q31;q22)x2, der(15)t(8;15)(q21;q25); carries IGH rearrangements and genomic MYC amplification; related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | BCBL-1 |
|---|---|
| 商品货号： | TS260111 |
| <strong>Cell line </strong>： | BCBL-1 |
| DSMZ no. ： | TS260111 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peritoneal effusion of a 40-year-old man (HIV+) with B-NHL (primary effusion lymphoma, PEL); cells were shown to carry the human herpesvirus type 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15854, 14483, 15200, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round/multiformed cells growing singly in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; immediately after thawing, viability might be rather low (60-70%) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum densitiy of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertetraploid karyotype with near-diploid and near-octaploid sidelines; 91-100<4n>XX, -Y, -Y, +2, +2, +6, +7, +7, +8, -9, -10, -11, +12, -13, -13, +15, -16, -22, -22, +2, -5mar, dup(1)(q12q44), del(1)(p11)dup(1)(q12q44), del(2)(p11)x1-2, der(2)t(2;5)(q35;?q32)t(5;8)(?q35;q22), der(4)t(4;8)(q24;q24), t(6;17)(p23;q21), del(9)(q12q21), der(11)t(11;15)(q23;q21), der(11)t(11;15)(q25;q25), der(13)t(13;13)(p12;q21)x1-2, der(14)t(4;14)(q31;q32)x2, der(14)t(14;22)(p12;q12)t(4;14)(q31;q22)x2, der(15)t(8;15)(q21;q25); carries IGH rearrangements and genomic MYC amplification; related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
