MINO
MINO
规格:
货期:
编号:TS260113
品牌:Testobio
| 产品名称： | MINO |
|---|---|
| 商品货号： | TS260113 |
| <strong>Cell line </strong>： | MINO |
| DSMZ no. ： | TS260113 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from peripheral blood mononuclear cells of a 68-year-old white woman with mantle cell lymphoma at relapse in 1997; described in the literature to carry a t(11;14)(q13;q32) leading to CCND1-IGH (BCL1-IGH) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15849, 15850, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat moded hypertriploid karyotype; 70-75<3n>XXY, -3, -5, +7, +14, -16, -16, -18, -19, +20, +20, -21, +4mar, del(3)(q13)x1-2, del(6)(22), i(7)(p10), der(8)t(8;14)(q24;q32)t(14;11)(q32;q13)x2, t(8;14)(q24;q32)x2, der(11)t(11;14)(q2?;q11)t(14;8)(q32;q24)x1-2, der(11)t(11;14)(q13;q32)x2, add(14)(q21), der(13)t(3;13)(q25;q32)x1-2; carries complex rearrangements of IGH with MYC and CCND1 (BCL1) consistent with MCL; although presence of Y-chromosome is inconsistent with published patient gender, karyotype otherwise resembles that published |
| Molec. Genetics ： | expression of fusion gene IGH-CCND1 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
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朱静
18067106081 -
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李晶晶
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周雪
13372109301
| 产品名称： | MINO |
|---|---|
| 商品货号： | TS260113 |
| <strong>Cell line </strong>： | MINO |
| DSMZ no. ： | TS260113 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from peripheral blood mononuclear cells of a 68-year-old white woman with mantle cell lymphoma at relapse in 1997; described in the literature to carry a t(11;14)(q13;q32) leading to CCND1-IGH (BCL1-IGH) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15849, 15850, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat moded hypertriploid karyotype; 70-75<3n>XXY, -3, -5, +7, +14, -16, -16, -18, -19, +20, +20, -21, +4mar, del(3)(q13)x1-2, del(6)(22), i(7)(p10), der(8)t(8;14)(q24;q32)t(14;11)(q32;q13)x2, t(8;14)(q24;q32)x2, der(11)t(11;14)(q2?;q11)t(14;8)(q32;q24)x1-2, der(11)t(11;14)(q13;q32)x2, add(14)(q21), der(13)t(3;13)(q25;q32)x1-2; carries complex rearrangements of IGH with MYC and CCND1 (BCL1) consistent with MCL; although presence of Y-chromosome is inconsistent with published patient gender, karyotype otherwise resembles that published |
| Molec. Genetics ： | expression of fusion gene IGH-CCND1 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
