MUTZ-8
MUTZ-8
规格:
货期:
编号:TS260125
品牌:Testobio
| 产品名称： | MUTZ-8 |
|---|---|
| 商品货号： | TS260125 |
| <strong>Cell line </strong>： | MUTZ-8 |
| DSMZ no. ： | TS260125 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1994 from the peripheral blood of a 63-year-old Caucasian woman with secondary acute myelomonocytic leukemia (sAML M4) 25 years after onset of myelodysplastic syndrom (MDS) and 2 years after chemotherapy with Ara-C; cells were described in the literature to carry JAK2 and TP53 mutations and a deletion of the 5q31 AML/MDS region effected by a t(5;11) translocation and to be constitutively cytokine-dependent |
| Reference(s) ： | 16046, 16047, 14871 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly in suspension; image; image; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10 ng/ml GM-CSF or 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35) |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 once or twice a week; after thawing cells may need some days to recover and a certain amount of cell debris may be allways visible; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | maximum density at ca. 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid/hypotetraploid karyotype; 71-92<4n>XXXX, -7, -7, -8, -9, -11, -12, -14, der(5)t(5;11)(q21;q10)x2, der(11;12)(p10;q10), der(17)t(10;17)(q11;q24)?dup(17)(p13pter)x2; matches published karyotype |
| Molec. Genetics ： | expression of mutated JAK2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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李晶晶
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周雪
13372109301
| 产品名称： | MUTZ-8 |
|---|---|
| 商品货号： | TS260125 |
| <strong>Cell line </strong>： | MUTZ-8 |
| DSMZ no. ： | TS260125 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1994 from the peripheral blood of a 63-year-old Caucasian woman with secondary acute myelomonocytic leukemia (sAML M4) 25 years after onset of myelodysplastic syndrom (MDS) and 2 years after chemotherapy with Ara-C; cells were described in the literature to carry JAK2 and TP53 mutations and a deletion of the 5q31 AML/MDS region effected by a t(5;11) translocation and to be constitutively cytokine-dependent |
| Reference(s) ： | 16046, 16047, 14871 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly in suspension; image; image; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10 ng/ml GM-CSF or 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35) |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 once or twice a week; after thawing cells may need some days to recover and a certain amount of cell debris may be allways visible; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | maximum density at ca. 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid/hypotetraploid karyotype; 71-92<4n>XXXX, -7, -7, -8, -9, -11, -12, -14, der(5)t(5;11)(q21;q10)x2, der(11;12)(p10;q10), der(17)t(10;17)(q11;q24)?dup(17)(p13pter)x2; matches published karyotype |
| Molec. Genetics ： | expression of mutated JAK2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
