KI-JK
KI-JK
规格:
货期:
编号:TS260128
品牌:Testobio
| 产品名称： | KI-JK |
|---|---|
| 商品货号： | TS260128 |
| <strong>Cell line </strong>： | KI-JK |
| DSMZ no. ： | TS260128 |
| Species ： | human (Homo sapiens) |
| Cell type ： | anaplastic large cell lymphoma |
| Origin ： | established in 1989 from the pleural effusion of a 15-year-old boy with Ki-1 positive lymphoma (anaplastic large cell lymphoma; ALCL); carries the translocation t(2;5) leading to NPM1-ALK fusion gene; cells were originally described in the literature to be EBV positive; however, viral analyses (PCR and western blot) at the DSMZ established that the cells do not harbor EBV |
| Reference(s) ： | 16263, 14464, 14492 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture; seed out at ca. 0.2-0.4 x 106 cells/ml (we recommend 12- or 24-well plates); cells grow slowly at first; viability may be low (60-70%) after thawing; later split culture 1:3 every 3-4 days and maintain at 0.1-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD4 -, CD14 -, CD15 +, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype; 75-82 <3n>XXYY, +1, +1, +2, +3, +6, +7, +8, -9, +16, -15, -17, +19, +20, +21, +2-3mar, add(Y)(q11), del(1)(p34)x2, der(1)t(1;5)(p11;q11)t(5;2)(q35;p21), der(1;16)(q10;p10), i(1)(q10)x2, t(2;5)(p23;q35)x1-2, add(4)(p15), add(9)(p22), der(15)add(15)(p11)dup(15)(q11q26)add(15)(q26), der(16)t(1;16)(q21;q13), der(19)t(7;19)(q12.2;q11), der(19)t(8;19)(p12;p13.1), add(21)(p11); sdl with del(7)(p12), add(9)(p21); carries t(2;5) effecting NPM1-ALK rearrangement |
| Molec. Genetics ： | expression of fusion gene NPM1-ALK confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | KI-JK |
|---|---|
| 商品货号： | TS260128 |
| <strong>Cell line </strong>： | KI-JK |
| DSMZ no. ： | TS260128 |
| Species ： | human (Homo sapiens) |
| Cell type ： | anaplastic large cell lymphoma |
| Origin ： | established in 1989 from the pleural effusion of a 15-year-old boy with Ki-1 positive lymphoma (anaplastic large cell lymphoma; ALCL); carries the translocation t(2;5) leading to NPM1-ALK fusion gene; cells were originally described in the literature to be EBV positive; however, viral analyses (PCR and western blot) at the DSMZ established that the cells do not harbor EBV |
| Reference(s) ： | 16263, 14464, 14492 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture; seed out at ca. 0.2-0.4 x 106 cells/ml (we recommend 12- or 24-well plates); cells grow slowly at first; viability may be low (60-70%) after thawing; later split culture 1:3 every 3-4 days and maintain at 0.1-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD4 -, CD14 -, CD15 +, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype; 75-82 <3n>XXYY, +1, +1, +2, +3, +6, +7, +8, -9, +16, -15, -17, +19, +20, +21, +2-3mar, add(Y)(q11), del(1)(p34)x2, der(1)t(1;5)(p11;q11)t(5;2)(q35;p21), der(1;16)(q10;p10), i(1)(q10)x2, t(2;5)(p23;q35)x1-2, add(4)(p15), add(9)(p22), der(15)add(15)(p11)dup(15)(q11q26)add(15)(q26), der(16)t(1;16)(q21;q13), der(19)t(7;19)(q12.2;q11), der(19)t(8;19)(p12;p13.1), add(21)(p11); sdl with del(7)(p12), add(9)(p21); carries t(2;5) effecting NPM1-ALK rearrangement |
| Molec. Genetics ： | expression of fusion gene NPM1-ALK confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
