OAC-M5.1
OAC-M5.1
规格:
货期:
编号:TS260130
品牌:Testobio
| 产品名称： | OAC-M5.1 |
|---|---|
| 商品货号： | TS260130 |
| <strong>Cell line </strong>： | OAC-M5.1 |
| DSMZ no. ： | TS260130 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from a lymph node metastasis of a 47-year-old white woman with esophageal adenocarcinoma in 1996 |
| Reference(s) ： | 16054, 16055 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing lightly adherent and in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; adherent cells can be detached by pipetting or tapping |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | OAC-M5.1 |
|---|---|
| 商品货号： | TS260130 |
| <strong>Cell line </strong>： | OAC-M5.1 |
| DSMZ no. ： | TS260130 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from a lymph node metastasis of a 47-year-old white woman with esophageal adenocarcinoma in 1996 |
| Reference(s) ： | 16054, 16055 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing lightly adherent and in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; adherent cells can be detached by pipetting or tapping |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
