ELF-153
ELF-153
规格:
货期:
编号:TS260131
品牌:Testobio
| 产品名称： | ELF-153 |
|---|---|
| 商品货号： | TS260131 |
| <strong>Cell line </strong>： | ELF-153 |
| DSMZ no. ： | TS260131 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established 1988 from a 41-year-old man with acute myeloid leukemia (AML-M7) during refractory relapse initially presenting with acute myelofibrosis. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16345, 16346, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; occasional semi adherent cells present; a few giant cells present; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 5 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.8-3 x 106 cells/ml; split culture 1:3 to 1:8 every 3-5 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | maximum density at ca. 5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy; 42(40-43)<2n>X, -Y, -7, -14, -17, del(5)(q13q32), der(6)t(6;7)(p23;p11), i(7)(q10), der(12)t(12;14)(p12;q11), add(20)(q13)x1-2; matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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ELF-153
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | ELF-153 |
|---|---|
| 商品货号： | TS260131 |
| <strong>Cell line </strong>： | ELF-153 |
| DSMZ no. ： | TS260131 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established 1988 from a 41-year-old man with acute myeloid leukemia (AML-M7) during refractory relapse initially presenting with acute myelofibrosis. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16345, 16346, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; occasional semi adherent cells present; a few giant cells present; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 5 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.8-3 x 106 cells/ml; split culture 1:3 to 1:8 every 3-5 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | maximum density at ca. 5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy; 42(40-43)<2n>X, -Y, -7, -14, -17, del(5)(q13q32), der(6)t(6;7)(p23;p11), i(7)(q10), der(12)t(12;14)(p12;q11), add(20)(q13)x1-2; matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
