| 产品名称: | SK-GT-2 |
|---|---|
| 商品货号: | TS260138 |
| <strong>Cell line </strong>: | SK-GT-2 |
| DSMZ no. : | TS260138 |
| Species : | human (Homo sapiens) |
| Cell type : | gastric fundus carcinoma |
| Origin : | established in 1989 from the primary tumor of a 72-year-old Hispanic man with a poorly differentiated adenocarcinoma of the gastric fundus; cells were described in the literature to be noninvasive using a Boyden chamber assay |
| Reference(s) : | 16343, 16344 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial cells growing adherently as monolayer |
| Medium : | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture : | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:3 twice a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV + |
| 产品名称: | SK-GT-2 |
|---|---|
| 商品货号: | TS260138 |
| <strong>Cell line </strong>: | SK-GT-2 |
| DSMZ no. : | TS260138 |
| Species : | human (Homo sapiens) |
| Cell type : | gastric fundus carcinoma |
| Origin : | established in 1989 from the primary tumor of a 72-year-old Hispanic man with a poorly differentiated adenocarcinoma of the gastric fundus; cells were described in the literature to be noninvasive using a Boyden chamber assay |
| Reference(s) : | 16343, 16344 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial cells growing adherently as monolayer |
| Medium : | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture : | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:3 twice a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV + |