FLO-1
FLO-1
规格:
货期:
编号:TS260141
品牌:Testobio
| 产品名称： | FLO-1 |
|---|---|
| 商品货号： | TS260141 |
| <strong>Cell line </strong>： | FLO-1 |
| DSMZ no. ： | TS260141 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established in 1991 from a primary tumor of a 68-year-old Caucasian man with esophageal adenocarcinoma |
| Reference(s) ： | 16055, 16056 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:5 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype; 82-89<4n>XXY, +1, -2, +7, +8, +8, -10, -10, -11, +16, +16, -17, -18, -18, +19, -20, +4mar; add(1)(q11), i(10)(q10), add(19)(q13), add(19)(q13)x2, add(20)(q13) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | FLO-1 |
|---|---|
| 商品货号： | TS260141 |
| <strong>Cell line </strong>： | FLO-1 |
| DSMZ no. ： | TS260141 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established in 1991 from a primary tumor of a 68-year-old Caucasian man with esophageal adenocarcinoma |
| Reference(s) ： | 16055, 16056 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:5 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype; 82-89<4n>XXY, +1, -2, +7, +8, +8, -10, -10, -11, +16, +16, -17, -18, -18, +19, -20, +4mar; add(1)(q11), i(10)(q10), add(19)(q13), add(19)(q13)x2, add(20)(q13) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
