OCI-LY18
OCI-LY18
规格:
货期:
编号:TS260143
品牌:Testobio
| 产品名称： | OCI-LY18 |
|---|---|
| 商品货号： | TS260143 |
| <strong>Cell line </strong>： | OCI-LY18 |
| DSMZ no. ： | TS260143 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1988 from the pleural fluid of a 56-year-old man with diffuse large B cell lymphoma (DLBCL, stage IIIB, at relapse); cells were described in the literature to be EBV negative and to carry a three-way translocation t(8;18;14) leading to alterations of MYC, BCL2, and IGH genes |
| Reference(s) ： | 14427, 16050, 16051 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-3.0 x 106 cells/ml; split culture 1:3-1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 3.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; 50(48-51)<2n>X, -Y, +9, +17, +18, +18, +18, der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), del(9)(q21.1), dup(12)(q11q15), der(14)t(14;18)(q32;q21), der(17)t(17;18)(p11.2;q21)t(18;14)(q21;q32), der(18)t(14;18)(q32;q21)x2, der(18)t(14;18)(q32;q21)t(14;8)(q32;q24)x2; carries complex series of two break IGH rearrangements involving MYC and BCL2 on ders(8)/(14)/(17)/(18); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | OCI-LY18 |
|---|---|
| 商品货号： | TS260143 |
| <strong>Cell line </strong>： | OCI-LY18 |
| DSMZ no. ： | TS260143 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1988 from the pleural fluid of a 56-year-old man with diffuse large B cell lymphoma (DLBCL, stage IIIB, at relapse); cells were described in the literature to be EBV negative and to carry a three-way translocation t(8;18;14) leading to alterations of MYC, BCL2, and IGH genes |
| Reference(s) ： | 14427, 16050, 16051 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-3.0 x 106 cells/ml; split culture 1:3-1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 3.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; 50(48-51)<2n>X, -Y, +9, +17, +18, +18, +18, der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), del(9)(q21.1), dup(12)(q11q15), der(14)t(14;18)(q32;q21), der(17)t(17;18)(p11.2;q21)t(18;14)(q21;q32), der(18)t(14;18)(q32;q21)x2, der(18)t(14;18)(q32;q21)t(14;8)(q32;q24)x2; carries complex series of two break IGH rearrangements involving MYC and BCL2 on ders(8)/(14)/(17)/(18); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
