IPEC-J2
IPEC-J2
规格:
货期:
编号:TS260145
品牌:Testobio
| 产品名称： | IPEC-J2 |
|---|---|
| 商品货号： | TS260145 |
| <strong>Cell line </strong>： | IPEC-J2 |
| DSMZ no. ： | TS260145 |
| Species ： | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type ： | intestine (jejunum) |
| Origin ： | established from normal intestinal epithelium cells isolated from the jejunum of a neonatal, unsuckled pig |
| Reference(s) ： | 16262, 16043, 16044 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently as monolayer; image; image |
| Medium ： | 90% Dulbecco´s MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:5 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | IPEC-J2 |
|---|---|
| 商品货号： | TS260145 |
| <strong>Cell line </strong>： | IPEC-J2 |
| DSMZ no. ： | TS260145 |
| Species ： | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type ： | intestine (jejunum) |
| Origin ： | established from normal intestinal epithelium cells isolated from the jejunum of a neonatal, unsuckled pig |
| Reference(s) ： | 16262, 16043, 16044 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently as monolayer; image; image |
| Medium ： | 90% Dulbecco´s MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:5 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
