KYAE-1
KYAE-1
规格:
货期:
编号:TS260149
品牌:Testobio
| 产品名称： | KYAE-1 |
|---|---|
| 商品货号： | TS260149 |
| <strong>Cell line </strong>： | KYAE-1 |
| DSMZ no. ： | TS260149 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from the pleural effusion of a 60-year-old Asian man with a distal non-Barrett-esophageal adenocarcinoma in 2001; the patient was pre-treated with radiation and chemotherapy; cells were shown to be tumorigenic in mice and to carry a P53 mutation (C455T) |
| Reference(s) ： | 16055, 16451 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer and small clusters |
| Medium ： | 40% Hams F12 + 40% RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; cells do not grow to confluency; split sub-confluent culture 1:2 to 1:5 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | KYAE-1 |
|---|---|
| 商品货号： | TS260149 |
| <strong>Cell line </strong>： | KYAE-1 |
| DSMZ no. ： | TS260149 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from the pleural effusion of a 60-year-old Asian man with a distal non-Barrett-esophageal adenocarcinoma in 2001; the patient was pre-treated with radiation and chemotherapy; cells were shown to be tumorigenic in mice and to carry a P53 mutation (C455T) |
| Reference(s) ： | 16055, 16451 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer and small clusters |
| Medium ： | 40% Hams F12 + 40% RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; cells do not grow to confluency; split sub-confluent culture 1:2 to 1:5 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
