| 产品名称: | OAC-P4C |
|---|---|
| 商品货号: | TS260152 |
| <strong>Cell line </strong>: | OAC-P4C |
| DSMZ no. : | TS260152 |
| Species : | human (Homo sapiens) |
| Cell type : | esophageal adenocarcinoma |
| Origin : | established in 1996 from the primary tumor of the gastro-esophageal junction (cardia) of a 55-year-old Caucasian man; the tumor was later classified as non-Barrett esophageal adenocarcinoma |
| Reference(s) : | 16054, 16055 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial cells growing adherently as monolayer; image |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:6 every 2-3 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 35-50 hours |
| Harvest : | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
| 产品名称: | OAC-P4C |
|---|---|
| 商品货号: | TS260152 |
| <strong>Cell line </strong>: | OAC-P4C |
| DSMZ no. : | TS260152 |
| Species : | human (Homo sapiens) |
| Cell type : | esophageal adenocarcinoma |
| Origin : | established in 1996 from the primary tumor of the gastro-esophageal junction (cardia) of a 55-year-old Caucasian man; the tumor was later classified as non-Barrett esophageal adenocarcinoma |
| Reference(s) : | 16054, 16055 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial cells growing adherently as monolayer; image |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:6 every 2-3 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 35-50 hours |
| Harvest : | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |