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OAC-P4C
OAC-P4C
规格:
货期:
编号:TS260152
品牌:Testobio
产品名称: OAC-P4C
商品货号: TS260152
<strong>Cell line </strong>: OAC-P4C
DSMZ no. : TS260152
Species : human (Homo sapiens)
Cell type : esophageal adenocarcinoma
Origin : established in 1996 from the primary tumor of the gastro-esophageal junction (cardia) of a 55-year-old Caucasian man; the tumor was later classified as non-Barrett esophageal adenocarcinoma
Reference(s) : 16054, 16055
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelial cells growing adherently as monolayer; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:6 every 2-3 days using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 35-50 hours
Harvest : cell harvest of ca. 8 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
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OAC-P4C

  • 货号: TS260152
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  • 品牌 : TESTOBIO
产品名称: OAC-P4C
商品货号: TS260152
<strong>Cell line </strong>: OAC-P4C
DSMZ no. : TS260152
Species : human (Homo sapiens)
Cell type : esophageal adenocarcinoma
Origin : established in 1996 from the primary tumor of the gastro-esophageal junction (cardia) of a 55-year-old Caucasian man; the tumor was later classified as non-Barrett esophageal adenocarcinoma
Reference(s) : 16054, 16055
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelial cells growing adherently as monolayer; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:6 every 2-3 days using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 35-50 hours
Harvest : cell harvest of ca. 8 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
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