MAVER-1
MAVER-1
规格:
货期:
编号:TS260170
品牌:Testobio
| 产品名称： | MAVER-1 |
|---|---|
| 商品货号： | TS260170 |
| <strong>Cell line </strong>： | MAVER-1 |
| DSMZ no. ： | TS260170 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood of a 82-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; mantle cell lymphoma) in 2003; described in the literature to carry the t(11;14) affecting the CCND1 (BCL1) and IGH genes |
| Reference(s) ： | 16453 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clumps in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; split culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human highly rearranged extreme hypodiploid karyotype with 10% polyploidy; 40(34-42)<2n>XY, -8, -12, -13, -14, -17, -22, der(1)dup(1)(p34p36)t(1;3)(p36;q21), der(3)del(3)(p13)t(3;14)(q27;q12), der(4)t(4;8)(q35;q24), der(6)t(6;14)(q13;q11)t(14;11)(q32;q13), der(8)dup(8)(q22q24)ins(8;7)(q24;?), ider(9)(q10)t(8;9)(q24;q34-35)t(8;22)(q24;q11), ider(9)del(9)(p21), der(10)t(8;10)(q13;p12), der(11)t(11;14)(q13;q32), der(12)t(12;12)(p13;q24)?del(12)(p11q11), der(14)t(3;14)(q13;q12), der(15)t(11;15)(p13;p11)dup(15)(q11q25), der(21)t(13;21)(q31;p11), der(22)t(18;22)(q22q23)dup(18)(q22q23)t(18;11)(q23;p13); resembles published karyotype; carries t(11;14) effecting rearrangement of IGH with CCND1 together with non-IGH rearrangements of MYC and amplification of IGH/CCND1 and BCL2 regions; karyotypically related but distinct sidelines present |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MAVER-1 |
|---|---|
| 商品货号： | TS260170 |
| <strong>Cell line </strong>： | MAVER-1 |
| DSMZ no. ： | TS260170 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood of a 82-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; mantle cell lymphoma) in 2003; described in the literature to carry the t(11;14) affecting the CCND1 (BCL1) and IGH genes |
| Reference(s) ： | 16453 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clumps in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; split culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human highly rearranged extreme hypodiploid karyotype with 10% polyploidy; 40(34-42)<2n>XY, -8, -12, -13, -14, -17, -22, der(1)dup(1)(p34p36)t(1;3)(p36;q21), der(3)del(3)(p13)t(3;14)(q27;q12), der(4)t(4;8)(q35;q24), der(6)t(6;14)(q13;q11)t(14;11)(q32;q13), der(8)dup(8)(q22q24)ins(8;7)(q24;?), ider(9)(q10)t(8;9)(q24;q34-35)t(8;22)(q24;q11), ider(9)del(9)(p21), der(10)t(8;10)(q13;p12), der(11)t(11;14)(q13;q32), der(12)t(12;12)(p13;q24)?del(12)(p11q11), der(14)t(3;14)(q13;q12), der(15)t(11;15)(p13;p11)dup(15)(q11q25), der(21)t(13;21)(q31;p11), der(22)t(18;22)(q22q23)dup(18)(q22q23)t(18;11)(q23;p13); resembles published karyotype; carries t(11;14) effecting rearrangement of IGH with CCND1 together with non-IGH rearrangements of MYC and amplification of IGH/CCND1 and BCL2 regions; karyotypically related but distinct sidelines present |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
