MEG-A2
MEG-A2
规格:
货期:
编号:TS260185
品牌:Testobio
| 产品名称： | MEG-A2 |
|---|---|
| 商品货号： | TS260185 |
| <strong>Cell line </strong>： | MEG-A2 |
| DSMZ no. ： | TS260185 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic myeloid leukemia |
| Origin ： | established 1995 from the peripheral blood of a 24-year-old man with chronic myelogenous leukemia (CML) in megakaryoblastic crisis after chemotherapy; cells are described in the literature to display several megakaryocytic features and to be inducible to megakaryocytic differentiation; cells carry the Philadelphia chromosome with an e14-a2 (b3-a2) BCR-ABL1 fusion gene |
| Reference(s) ： | 14484, 16448 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing singly in suspension; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; cells may grow slowly in the first week after thawing; split saturated culture 1:2 to 1:3 every 3-4 days; maintain at ca. 0.5-1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD19 -, CD33 +, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid/hypotriploid karyotype; 51-70<2n>XY, +3, +5, +8, +8, +8, +11, +13, +18, +19, -20, +21, +21, del(8)(q12)x2, der(8)t(8;20)(q12;q13)x2, der(9)t(9;22)(q34;q11), del(12)(p13), der(19)t(4;19)(q21;p13), der(19)t(19;20)(q13;p12), del(21)(q22.2)x2, der(22)t(9,22)x1-3; carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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李晶晶
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周雪
13372109301
| 产品名称： | MEG-A2 |
|---|---|
| 商品货号： | TS260185 |
| <strong>Cell line </strong>： | MEG-A2 |
| DSMZ no. ： | TS260185 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic myeloid leukemia |
| Origin ： | established 1995 from the peripheral blood of a 24-year-old man with chronic myelogenous leukemia (CML) in megakaryoblastic crisis after chemotherapy; cells are described in the literature to display several megakaryocytic features and to be inducible to megakaryocytic differentiation; cells carry the Philadelphia chromosome with an e14-a2 (b3-a2) BCR-ABL1 fusion gene |
| Reference(s) ： | 14484, 16448 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing singly in suspension; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; cells may grow slowly in the first week after thawing; split saturated culture 1:2 to 1:3 every 3-4 days; maintain at ca. 0.5-1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD19 -, CD33 +, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid/hypotriploid karyotype; 51-70<2n>XY, +3, +5, +8, +8, +8, +11, +13, +18, +19, -20, +21, +21, del(8)(q12)x2, der(8)t(8;20)(q12;q13)x2, der(9)t(9;22)(q34;q11), del(12)(p13), der(19)t(4;19)(q21;p13), der(19)t(19;20)(q13;p12), del(21)(q22.2)x2, der(22)t(9,22)x1-3; carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
